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Figure 1 | Molecular Cancer

Figure 1

From: miR-2909-mediated regulation of KLF4: a novel molecular mechanism for differentiating between B-cell and T-cell pediatric acute lymphoblastic leukemias

Figure 1

miR-2909 and KLF4 expression in pediatric acute lymphoblastic leukemias . (A) qRT-PCR analysis for relative expression of miR-2909 in patients with pediatric B-ALL and T-ALL compared with the corresponding controls. Expression was normalized to U6 snRNA; error bars represent mean ± S.D. n = 6, **P < 0.01 relative to B-and T-control. (B) Bioinformatics analysis of miR-2909 target site in the 3′UTR of KLF4 gene (C-D) KLF4 expression level at the mRNA (C) and protein (D) in patients with pediatric ALL compared with the corresponding controls. β-actin was used as an invariant control; each bar represents mean ± S.D of the experiment performed in triplicate *P < 0.05 relative to B-and T-control; densitometric analysis of western blot results was performed using Scion Image Analysis Software. (E-F) Melting curve graphs of hsa-miR-2909 (E) and KLF4 3′UTR (F) respectively in pediatric T-ALL. Difference between melting curve graph for KLF4 3′UTR in T-ALL and control is indicated by asterisk (*).

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Molecular Cancer

ISSN: 1476-4598