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Figure 3 | Molecular Cancer

Figure 3

From: miR-2909-mediated regulation of KLF4: a novel molecular mechanism for differentiating between B-cell and T-cell pediatric acute lymphoblastic leukemias

Figure 3

Sequence analysis of KLF4 coding region in B-ALL and T-ALL samples. (A) Snapshot of KLF4 gene sequence depicting the predominance of a splice variant (isoform 1) of KLF4 with the addition of 102 nucleotides between exons 3 and 4 in B-ALL samples. This entire region is absent in T-ALL samples. (B) Protein sequence depicting addition of 367-400 amino acids, confirming the presence of isoform 1 in B-ALL, while this entire region remains absent in all T-ALL samples in the present study (C) Presence of 1-50 amino acids confirms the existence of isoform 2 in all T-ALL study samples. The control sequence is shown for comparison. (D) Representative sequence data of pediatric T-ALL-1 sample is shown. A single nucleotide, T is inserted in the region corresponding to the third zinc-finger (Zf3) motif of KLF4 in T-ALL samples. (E) Genetic aberration(s) changed the entire reading frame, altering the sequence of KLF4 third zinc finger motif. The NCBI protein sequence is shown for comparison. Representative DNA and protein sequence alignment of KLF coding region (corresponding to three zinc finger motifs in exon 5) in other T-ALL samples are shown in Additional file 1: Figure S1.

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Molecular Cancer

ISSN: 1476-4598