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Figure 6 | Molecular Cancer

Figure 6

From: miR-2909-mediated regulation of KLF4: a novel molecular mechanism for differentiating between B-cell and T-cell pediatric acute lymphoblastic leukemias

Figure 6

Effect of miR-2909 knockdown on the expression level of various genes in pediatric patients with B-ALL and T-ALL. (A,C). qRT-PCR analysis of genes for KLF4, P21CIP, BCL3, MYC, AATF in B- (A) and T-lymphoblasts (C) transfected with antagomiR-2909 (50nM) compared with lymphoblasts transfected with control scrambled RNA (50nM). The data representation of mRNA expression levels of the above-mentioned genes in B- and T-lymphoblasts were compared with control B- and T-cells incubated for 48 h at 37°C in humidified 5% CO2 atmosphere. (B,D) KLF4 protein levels in antagomiR-2909 transfected B- (B) and T-lymphoblasts (D) compared with lymphoblasts transfected with scrambled RNA. Densitometric analysis of protein bands was carried out using Scion Image Analysis Software. Each bar represents mean ± S.D of the experiment performed in triplicate *P < 0.05 relative to control.

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Molecular Cancer

ISSN: 1476-4598