Skip to main content
Figure 1 | Molecular Cancer

Figure 1

From: HMGN2, a new anti-tumor effector molecule of CD8+ T cells

Figure 1

PHA induced HMGN2 expression in PBMCs. PBMCs were seeded at a density of 1 × 107 per well in 6 well plates and stimulated with 20 μg/ml PHA or 100 IU/ml IL-2 for 72 hours. Normal medium was used as the control. (A) The supernatants were collected and HMGN2 concentrations were analyzed by ELISA. (B) The cells were removed and stained with anti-CD3-PE and anti-HMGN2-IgG-FITC, followed to run on a Beckman coulter FC500 Flow cytometry. Data were analyzed by using Submit 5.2 software after gate lymphocytes (R5) group on dot plot graph. Figures are representative of three independent experiments. (C) Error bars represent HMGN2 intracellular expression positive rate (%) in CD3+ T cells. Data are represented as means ± SD of three independent experiments. *Significantly higher compared to medium control (p < 0.05). (D) Representative plots of the isotype staining and 2nd-Ab-FITC staining control.

Back to article page