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Figure 2 | Molecular Cancer

Figure 2

From: HMGN2, a new anti-tumor effector molecule of CD8+ T cells

Figure 2

PHA induced HMGN2 expression in different cell populations. PBMCs were seeded at a density of 1 × 107 per well in 6 well plates and stimulated with 20 μg/ml PHA or 100 IU/ml IL-2 for 72 hours. Normal medium was used as the control. Cells were collected and stained (A) Anti-CD4-PE surface stained and anti-HMGN2/IgG-FITC intracellular stained; (B) Anti-CD8-PE surface stained and anti-HMGN2/IgG-FITC intracellular stained. Figures are representative of three independent experiments. (C, D) Error bars represent HMGN2 intracellular expression positive rate (%) in CD4+ or CD8+ T cell populations. Data are represented as means ± SD of three independent experiments. *Significantly higher compared to IL-2 and medium control (p < 0.05), #Significantly higher compared to CD4+ T cell population (p < 0.05). (E) Representative plots of the isotype staining and 2nd-Ab-FITC staining control.

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