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Figure 5 | Molecular Cancer

Figure 5

From: Effective inhibition of melanoma tumorigenesis and growth via a new complex vaccine based on NY-ESO-1-alum-polysaccharide-HH2

Figure 5

Immunohistochemistry analyses were performed to study infiltrating lymphocytes (TILs) in tumors. A. Tumor tissue sections were separately incubated with anti-CD4, anti-CD8 and anti-CD57 mAbs overnight at 4°C. Sections were then incubated with biotinylated anti-mouse antibody and streptavidin-biotinylated peroxidase complex. B. Intensity of lympocyte infiltration in the tumors was determined by the mean positive cell counts in the dermis around the B16-NY-ESO-1 tumor per field (10 randomly selected high power fields/slide). For each site, 3 pathologists performed a blind read of the glass slides.

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Molecular Cancer

ISSN: 1476-4598