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Figure 6 | Molecular Cancer

Figure 6

From: Effective inhibition of melanoma tumorigenesis and growth via a new complex vaccine based on NY-ESO-1-alum-polysaccharide-HH2

Figure 6

PS-HH2 complex adjuvant promotes the antigen uptake and cross -presentation and maturation of DCs. A, DC were incubated with NHS-conjugated native NY-ESO-1 or protein adjuvant complex for 3 h, fixed, stained with DAPI and analyzed via confocal laser microscopy. B, DCs were incubated with Alexa Fluor-labeled NY-ESO-1 or NY-ESO-1/complex adjuvant for 3 h, fixed, permeabilized, blocked and stained with mouse FITC-anti-LAMP1 or FITC-anti-LMP2 antibody, respectively. DNA was stained with DAPI and analyzed via confocal laser microscopy. C, Analysis of surface maturation markers of DCs stimulated with PS-HH2 for 24 h.

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Molecular Cancer

ISSN: 1476-4598