Figure 7

Alum and PS-HH2 synergistically triggers activation of the NF-κB signal pathway. Immature DCs derived from bone marrow were incubated and harvested on Day 5 for Western blotting. After treatment with alum (125 μg/ml), PS-HH2 (PS: 20 μg/ml and HH2: 40 μg/ml), or alum-PS-HH2 complex for 0, 5, 10 and 30 min, respectively. DCs were washed, lysed and used for Western blot analysis using antibodies to phospho-Syk, phospho-Akt, phospho-IkBα and phospho-NF-κB, respectively. GAPDH was detected on the same membrane and used as the loading control.