Skip to main content
Figure 2 | Molecular Cancer

Figure 2

From: IL-1β-induced activation of p38 promotes metastasis in gastric adenocarcinoma via upregulation of AP-1/c-fos, MMP2 and MMP9

Figure 2

IL-1β upregulates MMP2 and MMP9 expression and activity by activating p38. A: RT-PCR analysis showed that MMP2 and MMP9 mRNA were upregulated in both AGS and MKN-45 cells in response to treatment with IL-1β; this effect was blocked by p38 siRNA or the p38 inhibitor SB202190. B: Quantification of the expression of MMP2 and MMP9 mRNA normalized to GAPDH mRNA. **P < 0.05 vs. scramble siRNA-transfected cells stimulated with IL-1β. △△P < 0.05 vs. untransfected cells stimulated with IL-1β. C: Gel zymography showed that MMP2 and MMP9 activity were upregulated in both AGS and MKN-45 cells in response to treatment with IL-1β; this effect was blocked by p38 siRNA or the p38 inhibitor SB202190. D: Immunocytochemical staining and confocal microscopy confirmed that IL-1β increased activation of p38 (red), and MMP2 and MMP9 (green) expression in MKN-45 GA cells; these effects were blocked by p38 siRNA or the p38 inhibitor SB202190.

Back to article page

Molecular Cancer

ISSN: 1476-4598