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Figure 3 | Molecular Cancer

Figure 3

From: IL-1β-induced activation of p38 promotes metastasis in gastric adenocarcinoma via upregulation of AP-1/c-fos, MMP2 and MMP9

Figure 3

IL-1β activates AP-1 activity through p38 pathway. A: Both GA cells were transfected with AP-1 reporter plasmid or AP-1 reporter plasmid together with scramble siRNA or p38 siRNA. AP-1 luciferase reporter gene activity was significantly increased by IL-1β stimulation in both AGS and MKN-45 cells; these effects were significantly inhibited by p38 siRNA in a dose-dependent manner and also inhibited by the p38 inhibitor SB202190. ** P < 0.05 vs. control siRNA (scramble siRNA) + AP-1 luc-transfected cells stimulated with IL-1β; ΔΔP < 0.05 vs. AP-1 luc-transfected cells stimulated with IL-1β; relative luciferase activity was normalized to B-gal. B: IL-1β-induced p38-mediated activation of the MMP9 promoters is dependent on the AP-1-binding sites. ** P < 0.05 vs. transfected −670/MMP9 + control siRNA cells stimulated with IL-1β; ΔΔP < 0.05 vs. transfected −670/MMP9 cells stimulated with IL-1β; ▼▼P < 0.05 vs. transfected −570/MMP9 cells stimulated with IL-1β; relative luciferase activity was normalized to B-gal.

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Molecular Cancer

ISSN: 1476-4598