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Figure 5 | Molecular Cancer

Figure 5

From: IL-1β-induced activation of p38 promotes metastasis in gastric adenocarcinoma via upregulation of AP-1/c-fos, MMP2 and MMP9

Figure 5

The activation of JNK is not associated with IL-1β-induced GA cell migration and invasion. A: p-JNK was detected by 30 min stimulation with IL-1β in AGS and MKN-45 cell lines. B: Transfection of JNK siRNA knocked down JNK expression in both the two GA cell lines. C-D: Treatment of GA cells with IL-1β increased cell migration and invasion in vitro; these effects were not inhibited by JNK siRNA nor the JNK pathway inhibitor SP600125. **P < 0.05 vs. scramble siRNA-transfected cells stimulated with IL-1β; P < 0.05 vs. untransfected cells (wild type cells) stimulated with IL-1β; ## or no significant different between these two groups was detected (P>0.05). Bars indicated the mean ± SD number of cells per field of view in the migration and invasion assays. D: Representative light microscope images of AGS and MKN-45 cell migration and invasion in the Transwell assays. E: Both GA cells were transfected with AP-1 reporter plasmid or AP-1 reporter plasmid together with scramble siRNA or JNK siRNA. AP-1 luciferase reporter gene activity was significantly increased by IL-1β stimulation in both AGS and MKN-45 cells; these effects were not inhibited by JNK siRNA nor inhibited by the JNK inhibitor SP600125 neither. ** P < 0.05 vs. control siRNA (scramble siRNA) + AP-1 luc-transfected cells stimulated with IL-1β; ΔΔP < 0.05 vs. AP-1 luc-transfected cells stimulated with IL-1β; ## or no significant different between these two groups was detected (P>0.05). Relative luciferase activity was normalized to B-gal.

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