Figure 6

p-c-Jun and p-STAT3 contribute to ISL-1 overexpression in NHL cells. (A to B) real-time RT-PCR (A) and Western blot (B) show the expression changes of ISL-1 in Raji and Ly3 cells after treated with JNK signaling pathway activator (Anisomycin, 15 ng/ml) or inhibitor (SP60012, 10 μM) for 6 h. (C to D) real-time RT-PCR (C) and Western blot (D) show the expression changes of ISL-1 in Ly3 cells after treated with JAK/STAT signaling pathway activator (IL-6, 4 ng/ml) or inhibitor (STATTIC, 6 μM) for 24 h. Each bar represents mean ± SD from three samples. p values were calculated using a Student t-test (*p < 0.05, vs. the control).