Figure 3

Analysis of transcriptional activity of the variant allele of the Rb1 promoter. a, CAT activity as measured by TLC chromatography of ROS17/2.8 rat osteoblast protein extracts following transient transfection with the BALB/c and the CBA variants of Rb1 promoter fused to CAT reporter constructs. b, Quantification of the CAT reporter signal driven by BALB/c (B) and CBA (C) promoter variants (positive orientation), CBA promoter (inverse orientation) and mock-transfected cells (negC). Transfection efficiency was normalized by simultaneous measurement of luciferase expression from co-transfected RFBpGL3 constructs