Figure 2

RAD001 inhibits EpS cell growth but increases AKT activation in vitro . A) Sensitivities of Asra-EPS, VAESBJ, and HDF cells to RAD001. Cells were exposed to various concentrations of RAD001 for 72 hours. Cell viability was determined by WST-1 assay. Points, mean; bars, SD. B) PI staining fluorescence-activated cell sorting analyses of the DNA contents of Asra-EPS and VAESBJ cells in response to RAD001. Cells were treated with 0.25–4 nM of RAD001 or vehicle for 24 hours. C) Effects of RAD001 on phosphorylation of S6RP and AKT in Asra-EPS and VAESBJ cells. Cells were treated with 0.25–4 nM of RAD001 or vehicle for 6 hours and with 1 nM RAD001 for 1–24 hours.