Figure 3

RAD001 delays Asra-EpS xenograft tumor growth but enhances AKT activation in vivo . A) Effects of RAD001 on Asra-EPS xenograft tumor growth. Nude mice bearing Asra-EPS xenograft tumors were treated with 5 mg/kg RAD001 (n = 5) or vehicle control (n = 5) thrice a week. Points, mean; bars, SD. *, p < 0.05, compared with control treatment. B) Asra-EPS xenograft tumor weights in control-treated and RAD001-treated mice. The average tumor weight recorded at termination of the study was 1613 ± 1200 mg in the control group and 466 ± 269 mg in the RAD001 group. Columns, mean; bars, SD. *, p < 0.05, compared with control treatment. C) Effects of RAD001 on S6RP phosphorylation in Asra-EPS xenograft tumors. Tumors were harvested 3 hours after the last administration and then cell lysates were prepared. D) Immunohistochemical staining of Ki-67 and p-AKT in control-treated and RAD001-treated Asra-EPS xenograft tumors. Scale bars: 100 μm. E) Ki-67-positivity rate of control-treated and RAD001-treated Asra-EPS xenograft tumors. Columns, mean; bars, SD. *, p < 0.05, compared with control treatment. F) Relative expression levels of p-AKT in control-treated and RAD001-treated Asra-EPS xenograft tumors. Relative expression levels were normalized against control-treated tumors. Columns, mean; bars, SD. *, p < 0.05, compared with control treatment.