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Figure 4 | Molecular Cancer

Figure 4

From: Combined targeting of mTOR and c-MET signaling pathways for effective management of epithelioid sarcoma

Figure 4

c-MET is highly activated through an autocrine mechanism in EpS, and c-MET activation affects EpS cell growth. A) Expression of phosphorylated RTKs in Asra-EPS and VAESBJ cells. B) Protein expression of c-MET and p-MET in Asra-EPS, VAESBJ, SYO-1, and HDF cells. C) HGF levels in cell-conditioned media and HGF serum concentrations in mice bearing xenograft tumors. Columns, mean; bars, SD. D) Expression of c-MET and p-MET in Asra-EPS and VAESBJ cells transfected with anti-c-MET siRNAs or a control siRNA. E) Proliferation of Asra-EPS and VAESBJ cells transfected with anti-c-MET siRNAs or a control siRNA. Cells transfected with siRNAs were cultured for 96 hours. Cell viability was determined by WST-1 assay every 24 hours. Points, mean; bars, SD. **, p < 0.01, compared with control. F) Colony formation of Asra-EPS and VAESBJ cells transfected with anti-c-MET siRNAs or a control siRNA. Columns, mean; bars, SD. **, p < 0.01, compared with control.

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Molecular Cancer

ISSN: 1476-4598