INC280 inhibits EpS cell growth in vitro , but Asra-EPS and VAESBJ cells differ from each other with regard to dependency on c-MET signaling. A) Sensitivities of Asra-EPS, VAESBJ, SYO-1, and HDF cells to INC280. Cells were exposed to different concentrations of INC280 for 72 hours. Cell viability was determined by WST-1 assay. Points, mean; bars, SD. B) PI staining fluorescence-activated cell sorting analyses of the DNA contents of Asra-EPS and VAESBJ cells in response to INC280. Asra-EPS and VAESBJ cells were incubated with 0.25–4 nM and 0.25–16 nM of INC280 for 24 hours, respectively. C) Effects of INC280 on caspase-3 cleavage in Asra-EPS and VAESBJ cells. Cells were treated with 0.25–64 nM of INC280 or vehicle for 24 hours. Staurosporine was used as a positive control. D) Effects of INC280 on phosphorylation of c-MET and its downstream effectors in Asra-EPS and VAESBJ cells. Cells were treated with 0.25–4 nM of INC280 or vehicle for 1 hour.