Figure 4

MiR-124 suppressed the lung and liver metastases of NPC cells in vivo . Groups of BALB/c nude mice were inoculated with lv-miR-124/5-8 F or lv-Ctrl/5-8 F cells. The liver and lung metastases were monitored by GFP-based fluorescence imaging on day 25 after inoculation (n = 5 per group). A, Lungs and livers were obtained from the mice of the lv-miR-124/5-8 F and lv-Ctrl/5-8 F groups. White arrows indicate liver and lung metastases. B, Representative H&E sections of liver and lung. Scale bars in lung, 200 μm; scale bars in liver, 2 mm. (C and D), Numbers of the metastatic nodules in the liver and lungs after injected with lv-miR-124/5-8 F or lv-Ctrl/5-8 F cell. Statistical analysis was performed using the nonparametric tests. *, P < 0.05, **, P < 0.01.