Figure 5

The ectopic miR-124 induced the expression of Foxq1 by directly targeting its 3′-UTR. A, Putative miR-124 binding site in the 3′-UTR region of Foxq1 and interspecies conservation of seed matching sequences (gray box). (B and C), The gene expression of Foxq1 in NPC cells compared with NP69 cells by RT-qPCR and western bolt. D, The protein expression level of Foxq1 in lv-miR-124/5-8 F cell and lv-miR-124/6-10B cell compared with control. E, Diagram of wt 3′-UTR and mut 3′-UTR of Foxq1 contained reporter constructs. F, Luciferase reporter assays in 5-8 F cells, co-transfected of wt/mut 3′-UTR with miRNAs as indicated. Statistical analysis was performed using the t-tests. The data represent the mean values of three independent experiments. *, P < 0.05, **, P < 0.01.