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Figure 6 | Molecular Cancer

Figure 6

From: Profilin-1 suppresses tumorigenicity in pancreatic cancer through regulation of the SIRT3-HIF1α axis

Figure 6

Pfn1 increases HIF1α protein degradation via SIRT3. (A-B) Effects of Pfn1 overexpression and Pfn1 KD on HIF1α protein level in MIA PaCa-2 cells under hypoxia. (C) Immunohistochemical (IHC) staining for Pfn1 and HIF1α in serial sections of clinical samples. (D) IHC staining for Pfn1 and HIF1α in slices of implanted tumors formed by the indicated cells (magnification, ×400). (E) Effect of transfected Pfn1 and SIRT3, either alone or in combination, on HIF1α levels in HEK-293 T cells under normoxia or hypoxia. (F) Effect of transfected Pfn1 and shSIRT3, either alone or in combination, on HIF1α levels in HEK-293 T cells under normoxia or hypoxia. (G) Schematic of the regulation of HIF1α by Pfn1. Error bars represent mean ± SD from three independent experiments. *: p < 0.05

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