G9a expression contributes to OCa cell adhesion, cell motility and anoikis resistance. A. Left panel: protein expression of G9a in OCa cell lines (ES-2, SKOV-3, TOV-21G, OV-90 and OVCAR-3), cyst cell line Hs832(c)T and two OSE primary cells. Right panel: Knockdown efficiency of two G9a specific shRNAs in SKOV-3 and ES-2 cells. B. Anoikis assays of SKOV-3 and ES-2 cells infected with specific shRNAs as indicated. C. Migration and invasion ability of G9a knock down cells. Wound-healing assays and transwell invasion assays were used to determine the cell migration and invasion ability of two OCa cell lines, SKOV-3 and ES-2. Reduced wound area after 24 h was analyzed by ImageJ analysis software. Cells that invaded into the opposite site of transwell were counted and presented as% of control. Representative micrographs of wound-healing assays shown above the plot. Scale bar: 500 μm. D. SKOV-3 cells with G9a depletion by shRNA that target 3’UTR region of G9a mRNA were transfected with long-form and/or short-form G9a and subjected to transwell invasion and HMC adhesion assays after fluorescence labeled with CellTracker™ Green CMFDA. Scale bar: 100 μm. Results indicated are mean values of three independently repeated experiments done in triplicate and are displayed as mean ± SD. *P < 0.05, **P < 0.01, indicate a statistically significance by one way ANOVA followed by Bonferroni test.