Knockdown of G9a inhibits peritoneal dissemination of OCa cells in vivo . A. SKOV-3 cells (1 × 106) were suspended in 100 μl PBS and intraperitoneally injected into severe combined immunodeficient (SCID) mice (6 mice/group). After 10 weeks of inoculation, the tumor burden and ascites formation in mice receiving control or G9a knockdown cells were estimated. Representative photos of tumor formation at the mesentery, diaphragm, peritoneum, and ascites are shown. Arrows: disseminated tumors. B. Box and whisker plot of the ascites volumes collected from the control and G9a knock down groups. C. Box and whisker plot of disseminated tumors in the abdominal cavities of the control and G9a knock down groups. D. The histological appearance of disseminated tumors in the abdominal cavities as indicated was assayed by H & E staining tissue from mice in the control and G9a knock down groups. T: tumor, arrows: invaded tumor mass. *P < 0.05, **P < 0.01, indicate a statistically significance by one way ANOVA followed by Bonferroni test.