Figure 1
From: Identification and characterization of Dicer1e, a Dicer1 protein variant, in oral cancer cells
Dicer1e is overexpressed in human OSCC cell lines of epithelial phenotype and in OSCC tissues. (A) Western blot analysis of Dicer1 and Dicer1e expression in a panel of human OSCC cell lines (CAL 27, SCC-4, SCC-9, SCC-15 and SCC-25) compared to normal human oral keratinocytes (HOK). The breast cancer cell line T47D was used as a positive control for Dicer1e protein expression based on the findings from the Hinkal et al. study [36]. Epithelial and mesenchymal phenotypes were determined by examining the expression of E-cadherin and vimentin. GAPDH was used as a loading control. The data are representative of three independent experiments. MWM, molecular weight markers (kDa). (B) Quantitative measurement of the relative fold expression levels of Dicer1 and Dicer1e proteins in oral cancer cell lines compared to HOK. Dicer1 and Dicer1e protein levels were normalized to GAPDH. Values are expressed as mean ± SEM of three independent experiments. (C) Western blot showing Dicer1e expression in SCC-4 and SCC-25 cells stimulated for 8 days with TGF-β compared to unstimulated cells. Epithelial-mesenchymal transition was confirmed by examining the expression of E-cadherin and vimentin. GAPDH was used as a loading control. (D) Western blot analysis of Dicer1 and Dicer1e protein expression in human adjacent normal (N) and tumor (T) tissues. GAPDH was used as a loading control.