Figure 2
From: Identification and characterization of Dicer1e, a Dicer1 protein variant, in oral cancer cells
5′ and 3′-RACE analyses confirm the presence of the Dicer1e transcript. (A) 5′-RACE analysis using a reverse primer (Dic1e5AS) designed to target a unique sequence found only in Dicer1e resulted in the amplification of a ~380 bp product that closely corresponded to the expected 5′-RACE product size of 373 bp. (B) 3′-RACE analysis followed by nested PCR using a forward primer (Dic1e3S2) designed to target a unique sequence of Dicer1e resulted in the amplification of a ~2,500 bp product that closely corresponded to the expected 3′-RACE product size of 2,472 bp. MWM, molecular weight markers.