Figure 4
From: Identification and characterization of Dicer1e, a Dicer1 protein variant, in oral cancer cells
The cellular localization of Dicer1e protein variant is predominantly nuclear. (A) Western blot analysis of Dicer1 and Dicer1e protein levels in nuclear (N) and cytoplasmic (C) fractions and whole (W) cell lysates of human OSCC cell lines (CAL 27, SCC-4, SCC-9, SCC-15, and SCC-25) and normal HOKs. The histone deacetylase class 1 (HDAC1) and GAPDH were used as nuclear and cytoplasmic markers, respectively. (B) HeLa cells transiently transfected with FLAG-Dicer1e. Representative images are shown of recombinant Dicer1e localization in cells (upper panels i-iii). Cells with predominantly nuclear (arrows), nuclear and cytoplasmic (arrowheads), or cytoplasmic (double-arrowheads) Dicer1e localizations are indicated. Nuclei were counterstained with DAPI (lower panels i-iii). Scale bar: 20 μm.