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Figure 1 | Molecular Cancer

Figure 1

From: Darpp-32 and t-Darpp are differentially expressed in normal and malignant mouse mammary tissue

Figure 1

Darpp-32 and t-Darpp expression in normal mouse mammary tissue. (A) Darpp-32 and t-Darpp protein levels were measured by Western analysis. β–Actin levels were measured as a loading control. Abdominal and inguinal mammary pads (#4/5 and #9/10) were collected from wild-type FVB mice and from premalignant transgenic mammary tumor mice. Mammary cells from two mice per strain (two fat pads per mouse) were analyzed. The ratio of Darpp-32 to t-Darpp (Dp32:tDp) protein expression was calculated for each sample. (B) Darpp-32 and t-Darpp RNA levels were measured by traditional RT-PCR using isoform-specific primers. β–Actin levels were measured as a loading control. RNA from a wild-type mouse brain was used as a positive control. Mammary tissue samples were: 1) NMuMG mouse mammary epithelial cell line, 2–5) four separate wild-type mammary pads (non-dissociated) from two mice and 6) dissociated wild-type mammary cells pooled from two mice.

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