Figure 2
From: Darpp-32 and t-Darpp are differentially expressed in normal and malignant mouse mammary tissue
Darpp-32 and t-Darpp expression in transgenic mouse mammary tumors. Nine individual tumors were collected from nine different MMTV-Neu mice. All ten MMTV-PyMT tumors were collected from two mice. Samples are arranged from smallest to largest (tumor volume) and the tumor location refers to the mammary pad from which the tumor originated. (A) Darpp-32 and t-Darpp protein levels were measured by Western analysis. α–Tubulin and β–Actin were used as loading controls. The ratio of Darpp-32 to t-Darpp (Dp32:tDp) protein expression was calculated for each sample. (B) Darpp-32 and t-Darpp RNA levels were measured by SYBR Green quantitative real-time RT-PCR using isoform-specific primers. Data was normalized to β–Actin; mean ± standard deviation.