Figure 5From: Clinical relevance and therapeutic potential of angiopoietin-like protein 4 in hepatocellular carcinomaANGPTL4 promoted apoptosis and suppressed angiogenesis of HCC. (A) TUNEL assay on liver tumor tissues. (B) Determination of microvessel density (MVD) of liver tumor tissues by immunohistochemical staining using anti-CD34 antibody. (C) Western blot analysis of VEGF, phosphor-Raf, Phospho-MEK, Phospho-Erk, ANGPTL4 and beta-actin proteins in liver tumor tissues in Ad-ANGPTL4 and Ad-Luc groups. The average protein expression between Ad-ANGPTL4 and Ad-Luc group was quantified and summarized in the lower panel figures. (D) Western blot analysis of proteins in MHCC97L cells after treating with rNGPTL4 protein. *, P < 0.05.Back to article page