Malignant features of five glioma cell lines. (A) H&E staining of the five glioma cell lines. (B) Representative images of GFAP expression in glioma cell lines by IHC staining. (C) Representative images of MMP-9 expression in glioma cell lines by IHC staining. (D) Representative images showing the invasive capability of the glioma cell lines. (E) Comparison of GFAP expression levels by IHC staining scores. (F) Comparison of MMP-9 expression by IHC staining scores. (G) Comparison of invasive capability by cell numbers. Values represent the mean ± SD in triplicate, and the data represent one of three independent experiments. One-way ANOVA was used, ***p < 0.001.