Figure 3
From: The chemokine receptor CXCR7 interacts with EGFR to promote breast cancer cell proliferation
CXCR7 ligand independent effect. A. MCF7 cells were stimulated with CXCR7 ligands CXCL11 (ITAC), CXCL12 (SDF-1a) and proliferation was evaluated. A significant increase in growth was observed in cultures treated with positive controls, estrogen (E2) or combination of estrogen with epidermal growth factor (E2 + EGF). B. Effect of endogenous and external added SDF-1a on MCF7 cell proliferation. Rescue of potential SDF-1a mediated autocrine activation of CXCR7 using siRNA to down regulate endogenous SDF1a (siSDF-1) compared to control siRNA (siC) and addition of external SDF-1a to media (+SDF-1); ns: no statistical difference. C. Percent survival of cells based on quantification of optical density measurements of cells treated 4-IPP, a specific MIF inhibitor for 48 hrs. MTT assay was used for viability estimation. D. Proliferation experiments comparing the stable line MCF7 vector shRNA control (shC) and shCXCR7 (shR7) treated with EGF (10 ng/mL) for 72 hrs. Data presented from one representative experiment with triplicate determination of cell numbers.