Figure 5

In vivo antitumor synergy between NVP-AEW541, MK2206 and BEZ235. Hep3B and Huh7 cells were subcutaneously injected into male BALB/c athymic nude mice. Mice were treated as indicated (NVP, NVP-AEW-541 30 mg/kg/day by gavage; MK2206, MK2206 100 mg/kg/week by intra-peritoneal injection; BEZ, BEZ235 25 mg/kg/day by gavage). Values presented are the means ± SD (n = 5 in each group). (A) Differences in tumor growth. (B) Differences in tumor cell apoptosis (TUNEL assay).*, p < 0.05; **, p < 0.01, compared with the control (vehicle-treated) group. (C) Difference in survivin measured by Western blotting of tumor lysates.