Figure 3
From: microRNA-29 negatively regulates EMT regulator N-myc interactor in breast cancer
miR-29 expression promotes mesenchymal plasticity. (A) Growth morphologies of T47D cells stably expressing miR-29 a or b were evaluated in tissue culture plates (2D growth) or in matrigel (3D growth). The results were documented as photomicrographs using phase contrast microscopy. (B) T47D miR-29a and miR-29b cells show loss of E-cadherin staining at the membrane as compared to scrambled controls. E- cadherin (red), DAPI (blue), and phase contrast images were taken at 20× (Scale bar =25 μm). (C) Quantitative real-time PCR of markers for EMT, E-cadherin, Slug, Snail, or Zeb1 of T47D miR-29a or b expressing cells. Data are normalized to GAPDH expression and fold changes (log2) in expression are relative to corresponding scrambled control. (D) Protein levels of epithelial markers, E-cadherin and Keratin 18 (KRT18) are reduced by T47D miR-29a or b expression.