TQ induces the phosphorylation of pPAKThr212and pPAKThr423in several colorectal cell lines. HCT116wt A, DLD-1 B, and HT29 C. cells were treated with 40 μM TQ and collected after 1, 3, 6 and 24 hours. Untreated cells were used as control. Whole cell lysates were immunoblotted against total PAK1, pPAKThr212, pPAKThr423 and pPAKSer144 (only for HCT116wt). Blots were then probed with GAPDH for loading control. Data shown are representative of two independent experiments.