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Figure 1 | Molecular Cancer

Figure 1

From: Tax impairs DNA replication forks and increases DNA breaks in specific oncogenic genome regions

Figure 1

Replication forks are slower in the presence of the HTLV-I Tax oncoprotein. (A) HTLV-I Tax expression was confirmed after induction with Cadmium chloride (CdCl) in Tax inducible Jurkat, JPX9 cells. (B). Induction of Tax expression was associated with an increase in DNA breaks detected by FACS analyses of p-H2AX. (C) Schematic representation of a single molecule analysis of DNA replication by molecular combing. (D) MT4, JPX9 and JPX9 Tax+ cells were pulse-labelled for 15 min with IdU then 15 min with CldU, and fibers were stretched by DNA combing. Blue: DNA. Red: IdU. Green: CldU. An example of a fiber representative for each condition is shown. (E) Replication fork speed analysis. The lengths of CldU tracks (ImageJ) were measured and divided by the length of the pulse (15 min). (F) Box and whisker plots: Box: 25–75 percentile range and Whiskers: 5–95 percentile range. More than 300 values coming from 3 independent experiments were compiled. Statistical analysis was performed using the non-parametric Mann–Whitney rank sum test. ns: not significant. *: p < 0.05; **: p < 0.01; ****: p < 0.0001.

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