Figure 4
From: Tax impairs DNA replication forks and increases DNA breaks in specific oncogenic genome regions
Reduced expression of Pol-H (A) and Pol-K (B) in HTLV-1 cell. Real-time PCR was performed in duplicate and samples were normalized to GAPDH expression. Fold change was calculated by comparing values with T cells (sorted from healthy donor) and/or Jurkat. (C) Viral tax mRNA expression was demonstrated by RT-PCR. Tax protein expression was detected by immunoblots in HTLV-I-transformed cell lines in vitro.