Skip to main content
Figure 5 | Molecular Cancer

Figure 5

From: Tax impairs DNA replication forks and increases DNA breaks in specific oncogenic genome regions

Figure 5

Tax expression increases DNA breaks and rearrangement in a human c-MYC promoter region rich in non-B-DNA forming sequences. (A) Establishment of stable cell lines containing a non-DNA sequence control from the GAPDH gene (pULCtrl), a c-MYC promoter region (pUMyc), and a BCL-2 gene major break region (pUMBR) as described in the Material and Methods. (B) pUCONT, pUMYC and pUMBR cell lines were infected with HRCMV or HRCMVTax virus for 48 h and analyzed by Flow cytometry to quantify ɣ-H2AX. Scatter plots are represented with ɣ-H2AX intensity on the y axis and propidium iodide intensity on the x axis. The cell populations used for the measurement of the ɣ-H2AX-positive cells were enclosed by rectangles. (C) Tax induces a small scale deletion in the c-MYC promoter. After 48 h infection by HRCMV or HRCMVTax, the genomic DNA was isolated from the pUMyc cells and subjected to PCR to amplify c-MYC promoter region. The PCR product was cloned into pGEM-T Easy vector and the inserts were verified by DNA sequencing.

Back to article page