Figure 1

Let-7d expression in RCC cell line and tumor samples, and correlation with tumor stromal cells. (A) Real-time RT-PCR analysis of relative let-7d expression in human cell lines. The data represent the average ± SD of three independent RT-PCR results. (B) The relative expression of let-7d in 80 RCC tissues and matched adjacent normal tissues were analyzed by real-time RT-PCR. (C, D) Real-time RT-PCR analysis of relative let-7d expression in 80 RCC tissues in different T stages (C) and tumor grades (D). Horizontal lines in (B–D) represent the mean values of relative let-7d expression for each series of samples. Note that (B–D) were generated from the same data as Table 1. (E) A linear regression and correlation among relative let-7d expression in a log scale vs. CD68 positive cell counts in five independent fields under × 20 objective lens is shown with r (Spearman) and P-values indicated.