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Figure 4 | Molecular Cancer

Figure 4

From: Let-7d suppresses growth, metastasis, and tumor macrophage infiltration in renal cell carcinoma by targeting COL3A1 and CCL7

Figure 4

Let-7d directly targets COL3A1 and CCL7 in RCC cells. (A) Sequence alignment of human let-7d seed sequence within the 3′-UTRs of COL3A1 and CCL7. The mutated sequence in the putative let-7d binding sites for each gene is shown in the top of each gene set. (B) Real-time RT-PCR analysis of COL3A1 and CCL7 expression in RCC cells. (C, D) The protein levels of COL3A1 and CCL7 in RCC cells were detected by western blot and ELISA. Corresponding densitometry of each band is presented in a bar graph. Data presented in (B–D) are the mean ± SD of three independent experiments. *P < 0.05. (E) IHC staining of COL3A and CCL7 expression in the tumor sections from each experimental group of PDX and CDX model. Original magnification: ×200. (F) Luciferase activity of various reporter plasmids. Statistical significance was obtained using one way ANOVA. The data represent the mean ± SD of three independent experiments with triplicates of each sample.*P < 0.05.

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