Figure 5From: Let-7d suppresses growth, metastasis, and tumor macrophage infiltration in renal cell carcinoma by targeting COL3A1 and CCL7Rescue of COL3A1 and CCL7 in RCC cells. (A) Proliferation assay was performed with or without the addition of 0.2 μg/mL type III collagen by CCK-8. (B) Representative images of the wound gaps in cells cultured with or without 0.2 μg/mL type III collagen at indicated time point. Original magnification: ×40. (C) Percent wound healing is shown as the mean ± SD of the three experiments. (D) Migrated PBMC cells were evaluated by chemotaxis assay with or without 10 ng/mL CCL7. Results are expressed as mean ± SD of three independent experiments. *P < 0.05.Back to article page