Figure 5
Survivin expression increased VEGF mRNA and VEGF protein levels in the medium: A,B: HEK293T cells (5×105) were seeded in 6-well plates and transfected with pEGFP-C1 or pEGFP-survivin (1.5 μg). A: After 24 h, VEGF (VEGF165 and VEGF121) and survivin mRNA levels were assessed by semi-quantitative RT-PCR. Actin was used as internal control. B: Total VEGF mRNA levels were assessed by qPCR. Ribosomal 18S RNA was used as internal control. C,D Extracellular VEGF was determined by an ELISA assay of supernatants from HEK293T (C) or MKN45 cells (D) transfected with pEGFP-C1 or pEGFP-survivin. Numerical data shown are the means ± s.e.m. of total VEGF measured in culture supernatants obtained in three independent experiments. Statistically significant differences compared to mock controls are indicated (* P < 0.05).