Figure 1
From: TopBP1 and Claspin contribute to the radioresistance of lung cancer brain metastases
Induction of radioresistant cells in vivo . (A) Clonogenic survival assays were performed with ionizing radiation (IR) treatment as indicated. Two lung cancer cell lines (PC14PE6 and H460) were exposed to IR and the survival was measured. (B) Cartoon shows the procedure for the generation of radioresistant lung cancer cells in lung cancer brain metastasis xenograft model. Each lung cancer cells (PC14PE6 and H460) were xenografted into a mouse brain to produce orthotopic metastasis model animals which were irradiated at various doses (H460, 5 and 10 Gy; PC14PE6, 15 and 20 Gy). (C-D) The H460 (C) and PC14PE6 (D) cells from the mouse brain were cultured in vitro, and exposed to various doses of radiation as indicated. After exposure to IR, cell survival was evaluated by cell proliferation assay (CCK-8 assay). Values are from at least three independent experiments and error bars represent the standard deviation. *P-value < 0.05; **P-value < 0.005.