Figure 1

MicroRNA-21 targets p57^Kip2gene. (A) miR-21 and p57^Kip2 are inversely correlated in 37 matched normal (N) and tumor (T) samples downloaded from the TCGA database. r: Pearson product-moment correlation coefficient. ****P < 0.0001 with one-way analysis of variance. (B) miR-21 (left panel) and p57^Kip2 (right panel) levels were measured in prostate cancer cells with real-time RT-PCR and western blotting respectively. *P < 0.05 with one-way analysis of variance test. (C) Sequence alignment between miR-21 seed sequence and a partial coding or mutated sequence of p57^Kip2. (D) Luciferase assay was performed with miR-21 mimic or anti-miR-21 transfection in cells with co-transfection of pMIR-3’UTR- p57^Kip2 or pMIR-3’UTR-mutated p57^Kip2 and a ß-Gal expression construct. **P < 0.01 with one-way analysis of variance and Tukey-Kramer post hoc test.