Figure 2

MicroRNA-21 downregulates p57^Kip2in prostate cancer cells. (A) 22Rv1 and MDA-PCa-2b cells were transfected with the inhibitor negative control and anti-miR-21 (50 nM) for 48 h. PC-3 cells were transfected with control siRNA and miR-21 mimic (20 nM) for 48 h. p57^Kip2 gene expression was checked by real-time RT-PCR. *P < 0.05 and ***P < 0.001 with a Student’s t-test analysis. (B) Cell lysates were used for Western analysis to measure the level of p57^Kip2 and GAPDH after anti-miR-21 inhibitor or miR-21 mimic transfection for 48 h. (C) p57^Kip2 and miR-21 expressions were analyzed in CWR22 tumors after 14 and 40 days of castration. ****P < 0.0001 with one-way analysis of variance test.