Figure 3

MicroRNA-21 attenuates p57^Kip2mediated functional responses in prostate cancer cells. Cells were transfected with 50 nM of control siRNA, anti-miR-21 or p57^Kip2 siRNA either alone or in combination for 48 h. Cells were trypsinized and replated for Western analysis, cell migration or soft agar assay. (A) Western analysis in PC-3 cells for p57^Kip2 and GAPDH was performed. (B) Number of cells migrated were quantified. *P < 0.05 and **P < 0.01 with one-way analysis of variance test. (C) After 14 days, colonies were stained and quantified in PC-3 and 22Rv1 cells for soft-agar colony formation assay. *P < 0.05, **P < 0.01 and ***P < 0.001 with one-way analysis of variance test.