Figure 3
From: Small nucleolar RNA 113–1 suppresses tumorigenesis in hepatocellular carcinoma
SNORD113-1 inhibited colony formation in culture and tumorigenesis. A. The expression of SNORD113-1 in HepG2 (□) and Huh7 (■) cells with or without infection of p3.1-SNORD113-1, SNORD113-1 siRNA or NC duplex. B. The effects of SNORD113-1 on cell viability in HepG2 cells. C. The effects of SNORD113-1 on colony formation in HepG2 cells. Left panel: the representative result of a colony formation assay; Right panel: the mean value from all three independent experiments determining colony formation. D. The effects of SNORD113-1 on xenograft tumor growth in nude mice. E. The effects of SNORD113-1 on cell cycle in HepG2 cells. F. The effects of SNORD113-1 on apoptosis in HepG2 cells. Upper panel: the results of apoptosis analysis measured with flow cytometry; Lower panel: the results of apoptosis analysis determined using caspase3/7 activity measurements. G. The effects of SNORD113-1 on cell migration and invasion in HCC cell lines. Upper panel: the representative result of cell migration and invasion; Lower panel: the mean value from at least three independent experiments of cell migration and invasion. *p < 0.05, **p < 0.01.