Figure 4

MAPK/ERK and TGF-β pathway were involved in SNORD113-1 effect. A, B. The relative reporter expression in HepG2 transfected with p3.1-SNORD113-1 (A) or SNORD113-1 siRNA (B) determined using Cignal Finder 10 Cancer Pathway Reporter Assays. HepG2 cells transfected with p3.1-SNORD113-1 or SNORD113-1 siRNA were seeded onto 96-well plates containing luciferase reporters to 10 common cancer pathways along with FuGene HD transfection reagent. Luciferase activity of the cells was measured using the dual luciferase assay system. Relative firefly luciferase activity was calculated and normalized to the constitutively expressed Renilla luciferase. C, D. The effect of SNORD113-1 on MAPK/ERK pathway (C) or TGF-β pathway (D). The expression and phosphorylation of key molecules of the pathways were detected using immunoblotting. GAPDH was used as an internal control.