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Figure 1 | Molecular Cancer

Figure 1

From: Transfection of poly(I:C) can induce reactive oxygen species-triggered apoptosis and interferon-β-mediated growth arrest in human renal cell carcinoma cells via innate adjuvant receptors and the 2-5A system

Figure 1

Poly(I:C) transfection can induce apoptosis in RCC cells. (a) RCC cells were cultured with poly(I:C) (white squares) or transfected with poly(I:C) (black circles) at the indicated doses. Cell viability was determined after 24 h using the WST-8 assay. Similar results were obtained from three independent experiments. (b) RCC cells were cultured or transfected with poly(I:C) at a dose of 1000 ng/ml for SKRC-1 cells and 500 ng/ml for SKRC-44 cells. Morphological changes were observed microscopically after 24 h. Magnification, ×40. Similar results were obtained from two independent experiments. (c) RCC cells were cultured or transfected with poly(I:C) (500 ng/ml for SKRC-1 and 50 ng/ml for SKRC-44). These cells were stained after 24 h with FITC-conjugated Annexin V and PI, and then analyzed by flow cytometry. Numbers represent the percentages of each subset. Similar results were obtained from five independent experiments. pIC, poly(I:C); pIC-TF, poly(I:C) transfection.

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Molecular Cancer

ISSN: 1476-4598