Figure 6

Participation of innate adjuvant receptors in antitumor effects of poly(I:C) transfected RCC cells. (a) RCC cells were transfected with RIG-1, MDA5, TLR3, or control siRNA, and selective knockdown was confirmed by immunoblot assay after 72 h. β-Actin was used as the control. (b) RCC cells, which were pre-transfected with the indicated siRNA 72 h before, were additionally transfected with poly(I:C) (125 ng/ml for SKRC-1 and 62.5 ng/ml for SKRC-44). Cell viability was determined by the WST-8 assay after 24 h. Similar results were obtained from two independent experiments. (c) The RCC cells were transfected with either IRF-3 or control siRNA, or selective knockdown was confirmed after 72 h by immunoblot assay. β-Actin was used as the control. (d) RCC cells, which were pre-transfected with the indicated siRNA 72 h before, were additionally transfected with poly(I:C) (250 ng/ml for SKRC-1 and 31.3 ng/ml for SKRC-44). Cell viability was determined by the WST-8 assay after 24 h. Similar results were obtained from two independent experiments. (e) RCC cells, which were pre-transfected with the indicated siRNA 72 h before, were additionally transfected with poly(I:C) (1000 ng/ml for SKRC-1 and 500 ng/ml for SKRC-44). The percentages of apoptotic cells were determined by flow cytometry after 24 h. Similar results were obtained from two independent experiments. pIC-TF, poly(I:C) transfection. *P < 0.05, **P < 0.01.