Figure 5

High expression of miR-196a and miR-196b in cancer tissues. A total of 54 paired cancer (T) and adjacent normal (N) tissues were obtained from patients with oral cancer. (A) For each sample, the expression levels of miR-196a and miR-196b were determined by RT-qPCR and normalized to U6 RNA as an internal control. (B) The relative expression of each sample is illustrated. The scatter dot plot shows the relative levels of miR-196a and miR-196b in the cancer and normal tissues from the patients. (C-D) Six paired of normal and oral cancer tissues were examined. (C) The expression level of miR-196a and miR-196b in the pairs of sample were determined by RT-qPCR based method. (D) The protein expressions of NME4, p-JNK and MMP9 were determined by western blot method, and showing GAPDH level as an internal control. Relative expressions of NME4, pJNK and MMP9 in each paired tissues. The density of each protein band was quantified after normalization to the GAPDH in each sample. The lines indicate the tendency of altered expressions of NME4, pJNK and MMP9 in each paired tissues. (*p < 0.05, **p < 0.01, ***p < 0.005, t-test).