Screen for SAP-dependent Mkl1 target genes and their implication in cancer. (A) Scatter plot and (B) Venn diagram representing classification of Mkl1 target genes into three groups: SRF-dependent/SAP-independent (blue), SRF-dependent/SAP-dependent (red) and SRF-independent/SAP-dependent (green). The scatter plot (A) represents the log fold change (logFC) in gene expression in HC11-∆SAP versus HC11-FL control cells (x-axis; ∆SAP vs. FL) and between HC11-mutB1 versus HC11-FL control cells (y-axis; mutB1 vs. FL). Each dot represents a probeset, and the one for tenascin-C is highlighted (Tnc). The vertical and horizontal lines in the chart denote the 2-fold change cutoff (logFC = -1). The Venn diagram (B) represents the number of probesets for transcripts, which are more than 2-fold reduced in either HC11-mutB1 or HC11-ΔSAP cells when compared to HC11-FL control cells. Boxes below the Venn diagram indicate the cell strains that have reduced levels of the respective transcripts. (C, D) Functional analysis for the three Mkl1-regulated gene sets performed using the IPA software. The high-level functional (C) and disease (D) categories are displayed along the x-axis of each bar chart. The y-axis displays the –log of the P-value determined by right-tailed Fisher’s exact test. The P-value is a measure of the likelihood that the association between a set of genes in each dataset and a related function or disease is due to random association. The grey vertical line denotes the cutoff for significance (P = 0.05; -logP = 1.3).